Applications:Suitable for use in immunoblot (western or dot blot), ELISA and immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA (standard capture): 1:500-1:1500, 1.0 ug of Horse IgG using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:goat
Source Antibody:equine
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography
Form:Supplied as a liquid in TBS, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% Glycerol; pH 8.0, 10 mg/ml BSA (IgG and Protease free), 0.01% Sodium Azide.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Horse IgG and Horse Serum.