Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins.
Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis.
The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site.
Both heavy and light chains of the antibody molecule are present.
Secondary Antibodies are available in a variety of formats and conjugate types.
When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:20,000-1:60,000Western Blot: 1:2000-1:20,000Immunohistochemistry: 1:1000-1:5000Optimal dilution determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2mg
Host：goat
Source Antibody：equine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS pH 7.2, 10mg/ml BSA, (IgG and Protease free), 0.01% gentamicin sulfate. Reconstitute with 1ml sterile dH2O. Labeled with Horseradish Peroxidase (HRP).
Specificity：Recognizes equine IgG, H&L. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, equine IgG and equine serum.
