84-1068-14 IgG, H&L (Biotin) 2mg I1903-21K
特徴
Applications: Suitable for use in immunoblot, ELISA, immunohistochemistry and immunomicroscopy.
Other applications not tested.
Recommended Dilution:ELISA (standard capture): 1:20,000-1:100,000, 1.0 ug of Horse IgG using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Optimal dilution determined by the researcher.
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2mg
Host：goat
Source Antibody：equine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 10 mg/ml BSA (IgG and Protease free), 0.01% Sodium Azide, 40% glycerol
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Horse IgG and Horse Serum.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Eq
Immunogen：horseIgG whole molecule
Shelf Life：1year
EU Commodity Code：30021010
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