Applications:Suitable for use in immunoblot, ELISA, immunohistochemistry, immunomicroscopy.
Other applications not tested.
Recommended Dilution:ELISA (standard capture): 1:3,000-1:15,000, 1.0 ug of Horse IgG using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1.5mg
Host：rabbit
Source Antibody：equine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10 mg/ml BSA,(IgG and protease free), 0.01% sodium azide, 40% glycerol.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Horse IgG, Horse IgG F(c) and Horse Serum. No reaction was observed against Horse IgG F(ab’)2.
