Applications:Suitable for use in Immunoblot, ELISA, immunohistochemistry, immunomicroscopyOther applications not tested.
Recommended Dilution:ELISA (standard capture): 1:40,000-1:130,000, 1.0 ug of Horse IgG using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2mg
Host：goat
Source Antibody：equine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 10 mg/ml BSA, (IgG and Protease free), 0.01% Thimerisol, 40% glycerol.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin,anti-Goat Serum, Horse IgG, Horse IgG F(c) and Horse Serum. No reaction was observed against Horse IgGF(ab’)2.
