Applications:Suitable for use in Western Blot, ELISA and immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:20,000-1:130,000; 1ug of Canine IgG was used in a standard capture ELISA with Streptavidin (HRP) (S7973-97A) and ABTS (A0030) as a substrate for 30 minutes at RT
Immunohistochemistry: 1:1000-1:5000Western Blot: 1:2000-1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：goat
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin.
Specificity：Recognizes canine IgG, Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, canine IgG, canine IgG F(c) and canine serum. No reaction was observed against canine IgG F(ab’)2.
