Applications:Suitable for use in Immunofluorescence and FLISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Absorption 4avelength: 495nm.
Emission Wavelength: 528nm.
Fluorochrome/Protein Ratio: 4.0moles FITC per mole of Donkey IgG.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:donkey
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder in PBS, pH 7.2, 0.09% sodium azide, 1% BSA. Label: Fluorescein Isothiocyanate (MW 390D) (FITC). Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.