Applications:Suitable for use in Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy Other applications not tested.
Recommended Dilution:ELISA: 1:20,000 to 1:100,000.
1.0 ug of Cat IgG was used in a standard sandwich ELISA with Streptavidin (HRP) (S7973-97A) and ABTS Stable Liquid Substrate 1.46mM/Liter for HRPO (A0030) as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2mg
Host：goat
Source Antibody：feline
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC).
Specificity：Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, feline IgG, feline IgG F(ab’)2 and feline Serum. No reaction was observed against feline IgG F(c).
