IgA is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas.
The most common feature of these malignancies is the restricted expression of a single heavy chain class.
Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Applications:Suitable for use in FLISA and Immunofluorescence.
Other applications not tested.
Recommended Dilution:FLISA: 1:10,000-1:50,000Immunofluorescence Microscopy: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Absorption Wavelength: 596nm
Emission Wavelength: 620nm
Fluorochrome/Protein Ratio:3.0 moles Texas Red per mole of Rabbit IgG
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer. Texas Red® conjugates are sensitive to light.
仕様
Size：1mg
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Texas Red™ Sulfonyl Chloride (MW: 625 daltons). Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes mouse IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, mouse IgA and mouse serum. Specificity was confirmed by ELISA at <1% against other mouse heavy or light chain combinations.
