84-1067-15　Hypoxia Inducible Factor 3a (HIF-3a) 100ug　H9810-05

特徴

• One of the most important factors in the cellular response to hypoxia is hypoxiainduciblefactor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability.
• HIF is a heterodimer consisting of one of three subunits (HIF1-a, HIF2-a, or HIF3-a) bound to the aryl hydrocarbon receptor nuclear translocator (ARNT), which is also known as HIF1-ß.
• HIF-a is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerizationwhile the basic domain binds to DNA.
• HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival; in fact, HIF3-a may be a marker for tumor growth and angiogenesis .
• Examples of HIF target genes include VEGF, glucose transporter 1 (GLUT1), and EPO.
• HIF binds to the hypoxia-responsive element, which contains the core recognition sequence 5'-TACGTG-3', in the cis-regulatory regions of hypoxiainducible genes.
• Transcriptional activation by HIF is linked to its ability to recruit coactivator proteins such asCREB-binding protein (CBP), p300, steroid receptor coactivator-1, and translation initiation factor 2.
• Hif3a may be a marker for tumor growth and angiogenesis.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:7000-1:30,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.