Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.
Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.
Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.
This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means.
This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA.
Also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:50,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes His-Tag (6-Histidine). Detects over-expressed proteins containing the 6X His epitope tag. Reacts with all His-tagged proteins so far tested. In Western Blot of bacterial extracts the antibody does not crossreact with endogenous proteins. Recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Immunogen：6-His epitope tag peptide (HHHHHH), conjugated to KLH using maleimide
