Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation.
Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells.
GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages.
GM-CSF receptors show significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.
Applications:Suitable for use in ELISA, Western Blot and Neutralization.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:5000 Western Blot: 1:500-1:2000Neutralization: 1:400 Recommended incubation of at least 4 hours
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum. Endotoxin: ≤10pg/ul (LAL)
Form：Supplied as a liquid. No stabilizing proteins or preservatives added.
Specificity：Recognizes native and recombinant human GM-CSF in cell supernatants and certain body fluids.
Isotype：IgG
Calc Applications Abbrev：E N WB
Calc Crossreactivity：Hu
Immunogen：Full length recombinant human Granulocyte-Macrophage Colony Stimulating Factor.
