Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase.
Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology.
GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
GSK 3B participates in the Wnt signaling pathway and has been implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
GSK 3B phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:200-1:1000Western Blot: 1:500-1:3000Immunofluorescence: 1:200-1:1000ELISA: 1:10,000-1:50,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as liquid in PBS, pH7.2, 0.01% sodium azide.
Specificity：Recognizes human phosphorylated human GSK3B, pS9. Assay by IEP resulted in a single precipitin arc against anti-Rabbit Serum. Minimal reactivity occurs against non-phosphorylated GSK3B.
Isotype：IgG
Calc Applications Abbrev：E IF IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the N-Terminal region near aa1-15 from human GSK3 beta.
