Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units.
Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000.
GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form.
GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase.
Phosphorylation of GS at S640 has been associated with Antiphospholipid Antibody Syndrome.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilutions:ELISA: 1:20,000 - 1:60,000Western Blot: 1:1000-1:10,000Immunohistochemistry (paraffin): 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Specificity：Recognizes human Glycogen Synthase 1 (Muscle) (GYS1). Species Crossreactivity: Mouse. Minimally reactive (less than 1%) with the non-phosphorylated form of the immunizing peptide.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phospho peptide corresponding to a S641 region of human Glycogen Synthase, conjugated to KLH.
