GST (Glutathione S-Transferase) is a 26kD protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
Applications:Suitable for use in Western Blot, ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:50,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilution determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2,10 mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin.Reconstitute with 1m sterile dH2O.
Specificity：Recognizes Glutathione-S-Transferase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
