Applications:Suitable for use in ELISA, Immunoprecipitation and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000-1:20,000.
1ug of Glutamine Synthetase was used in a standard capture ELISA with Streptavidin (HRP)( S7973-97A) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) A0030 as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography prior to conjugation to biotin.
Form：Supplied as powder lyophilized from a solution containing PBS (pH 7.2), 10mg/ml BSA and 0.01% sodium azide.
Specificity：Recognizes Glutamine Synthetase. Target species: microbial. Known reactivity: Brevibacterium. Assay by IEP resulted in a single precipitin arc against anti-Biotin, anti-goat serum as well as purified and partially purified Glutamine Synthetase.
