Applications:Suitable for Immunoblot, ELISA, Immunohistochemistry, Immunomicroscopy.
Other applications not tested.
Recommended Dilution:ELISA: 1:8000-1:34,000; 1.0ug of Fructosyl Amino Acid Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio:10-20 BAC molecules per Sheep IgG molecule
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Grade：Highly Purified
Purity：Purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
Form：Supplied as a liquid in PBS, pH 7.2, 10 mg/ml BSA, IgG and Protease free, 0.01% Sodium Azide, 40% glycerol.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Fructosyl Amino Acid Oxidase [E. coli].
