Applications:Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation andmost immunological methods.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:8000 1ug of Fructosyl-Amino Acid Oxidase (E.coli) was used in a standard sandwich ELISA with Rabbit anti-Sheep (HRP) and ABTS Stable Liquid Substrate 1.46mM/Liter for HRPO A0030 as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：sheep
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Assay by IEP resulted in a single precipitin arc against anti-sheep serum as well as purified and partially purified Fructosyl-Amino Acid Oxidase (E. coli).
