Fibrinogen is an abundant plasma protein (5-10uM) produced in the liver.
The intact protein has a molecular weight of 340kD and is composed of 3 pairs of disulfide-bound polypeptide chains named Aalpha, Bbeta and gamma.
Fibrinogen is a triglobular protein consisting of a central E domain and terminal D domains.
Proteolysis by thrombin results in release of Fibrinopeptide A (FPA, Aa1-16) followed by Fibrinopeptide B (FPB, Bb1-14) and the fibrin monomers that result polymerize in a half-overlap fashion to form insoluble fibrin fibrils.
The chains of fibrin are referred to as a, b and g, due to the removal of FPA and FPB.
The polymerized fibrin is subsequently stabilized by the transglutaminase activated Factor XIII that forms amide linkages between g chains and, to a lesser extent, a chains of the fibrin molecules.
Proteolysis of fibrinogen by plasmin initially liberates C-terminal residues from the Aa chain to produce fragment X (intact DE- D, which is still clottable).
Fragment X is further degraded to nonclottable fragments Y (D-E) and D.
Fragment Y can be digested into its constituent D and E fragments.
Digestion of non-crosslinked fibrin with plasmin is very similar to the digestion of fibrinogen, which results in production of fragments D and E.
Degradation of crosslinked fibrin by plasmin results in fragment DD (D-Dimer consisting of the D domains of 2 fibrin molecules crosslinked via the y chains), fragment E (central E domain) as well as DDE in which fragment E is non-covalently associated with DD.
For human crosslinked fibrin, the relative weights of the cleavage fragments produced are: 184kD for fragment DD, 92kD for D, 50kD for E, 1.54kD for FPA and 1.57kD for FPB.
Applications:Suitable for Immunoblot (Western or Dot Blot), ELISA, Immunomicroscopy and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:50,000-1:250,000; 1ug of Fibrinogen was used in a standard capture ELISA with Streptavidin (HRP), Cat # S7973-97A, and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]), Cat # A0030 as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio:10-20 BAC molecules per Goat IgG molecule
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography.
Form：Supplied as a lyophilized power from PBS, pH 7.2, 10mg/ml BSA, 0.09% sodium azide. Labeled with Biotin. Reconstitute with 100ul ddH2O.
Specificity：Recognizes human Fibrinogen. Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified fibrinogen (human plasma).
