84-1066-80　［取扱停止］DNA Methyltransferase 3L (Dnmt 3L, DNA Cytosine 5’ Methyltransferase 3 Like Protein) 100ug　D3931-76

特徴

• DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands.
• CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation.
• Studies in mice havedemonstrated that DNA methylation is required for mammalian development.
• This gene encodes a nuclear protein with similarity to DNA methyltransferases.
• This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity.
• However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints.
• This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
• Applications:Suitable for use in ELISA, Immunoprecipitation and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1000-1:3000Western Blot: 1:1500.
• Detects human DNMT3L by Western Blot after Immunoprecipitation.
• Transfection of U2OS cells(10cm dish) was accomplished using 3ug of GAL4-DNMT3L.
• Protein extraction using IPH at 150mM.
• Does not perform well when applied directly for Western Blot.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50% ) glycerol, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.