The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins.
This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome.
Phosphorylation by cAMPdependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II.
Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:2000.
Detects bands of 46 and 43kD corresponding to phosphorylated CREB
Immunohistochemistry (FFPE): 20ug/mlELISA: 1:1000-1:6000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Specificity：Recognizes phosphorylated human CREB at pS133. Species Crossreactivity: mouse and rat. Minimal reactivity with non-phosphorylated CREB by Western Blot and minimal reactivity (1%) by ELISA against the non-phosphorylated form of the immunizing peptide. Assayed against a variety of tisues including fibroblasts and B-cell lysates.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa122-147, QKRREILSRRPpSYRKILNDLSSDAP, from human CREB Phosphorylated at pS133 (KLH).
