COX-2, also known as prostaglandin H synthase, was identified less than a decade ago.
Its discovery was followed by a period of discovery and drug development to create a “super aspirin” and led to insights into arthritis, Alzheimer's disease, colorectal cancer and regulation of brain and kidney function in search of better treatments for degenerative and inflammatory diseases.
Two different isoforms of the enzyme are known, Cox-1 and Cox-2.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1:5000-1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Purified by delipidation and defibrination.
Form：Supplied as liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes Cox-2 at 70kD in Sf9 cell lines transfected with Cox-2 and in WISH cells induced with IL-1b. Does not cross react with Cox-1. Species Crossreactivity: Human, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A GST fusion protein, the epitope of which maps to the carboxy- terminus of human Cox-2.
