Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:4000-1:20,000 1ug of Carbonic Anhydrase II was used in a standard capture ELISA with Streptavidin (HRP) and ABTS (A0030) as a substrate for 30 minutes at RT
Western Blot: 1:200-1:10,000
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), and 0.01% sodium azide, before the addition of glycerol to 40%. Labeled with Biotin.
Specificity：Assay by IEP resulted in a single precipitin arc against anti-Biotin, anti-rabbit serum as well as purified and partially purified Carbonic Anhydrase II (Human Erythrocytes).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Carbonic Anhydrase II (Human Erythrocytes).
