Applications:Suitable for use in FLISA and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Excitation Wavelength: 650nm
Emission Wavelength: 667nm
Fluorochrome/Protein Ratio: 5.3 moles Cy5 per mole of goat IgG F(ab’)2.
Molecular weight of label: 975D.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:goat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Cy5. Reconstitute with 1ml sterile ddH2O.
Specificity:Recognizes a single precipitin arc against anti-goat serum, biotin conjugated goat IgG and biotin conjugated BSA. No reaction was observed against anti-goat IgG F(c) or anti-pepsin.
Isotype:F(ab’)2
Calc Applications Abbrev:FLISA IF WB
Calc Crossreactivity:Gt
Immunogen:Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).