AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308.
AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases).
AKT is a key regulator of many signal transduction pathways.
AKT Exhibits tight control over cell proliferation and cell viability.
Overexpression or inappropriate activation of AKT is noted in many types of cancer.
AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin).
PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation.
Oncephosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.
AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence (paraformaldehyde): 1:100-1:10,000ELISA: 1:2000-1:10,000Western Blot: 1:500-1:2000Immunohistochemistry (Formalin, paraffin): 1:250-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human AKT.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa460-480, CRPHFPQFSYSASGTA, from the C-terminus of human AKT proteins (KLH). Species sequence homology: rat, mouse and chicken AKT.
