Kinase of embryonic stem cells (Kos1) is a nonreceptor protein tyrosine kinase (NRPTK) and a new member of the Ack family of NRPTKs.
Kos1 is a ubiquitously expressed 47-kD protein with autotyrosine kinase activity that is developmentally regulated during embryogenesis.
Kos1 is also upregulated following IL3 withdrawal from factor-dependent murine NSF/N1.H7 cells that undergo apoptosis, suggesting a role in growth inhibition.
Data support a negative regulatory role for Kos1 in regulating the Ras-Raf1-MAPK growth pathway by a mechanism that requires its autotyrosine kinase activity.
Applications:Western Blot: Use at 1:500-1000 dilution.
However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Reacts with the C-terminal residues 421-434 [QNQVQSLPAGSAEP] of the 47kD mouse Kos1 protein.
