DNA fragmentation is one of the critical steps in apoptosis, which is induced by DNA fragmentation factor (DFF).
DFF is composed of two subunits, a 40kD caspase-activated nuclease (DFF40) and a 45kD inhibitor (DFF45).
Recently a novel family of cell-death-inducing DFF45-like effectors (CIDEs) has been identified.
Human CIDE-3 is a novel member of CIDEs.
There are 2 transcripts, CIDE-3 and CIDE-3alpha, present in HepG2 and A375 cells.
Consistent with its chromosome localization at 3p25, a region associated with high frequency loss of heterozygosity in many tumors, CIDE-3 may play an important role in prevention of tumorigenesis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1000Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes residues 10-25 [LLYPKSLSRHVSVRTS] of the 27kD human CIDE-3 protein. Crossreactivity to FSP27, which is the mouse homolog of CIDE-3: 93% sequence identity.
