Salmonella is a genus of the family Enterobacteriaceae populated by a variety of Gram negative rod-shaped bacteria, many of which are pathogenic and cause a range of diseases in humans.
Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae).
Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria.
This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A.
Applications:Suitable for use in Immunofluorescence.
Direct FA staining of target antigens in a permissive tissue culture system.
Acetone fixation of the antigen source is recommended prior to staining.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：rabbit
Grade：Highly Purified
Purity：Purified IgG fraction to a purity of > 95%.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.09% sodium azide. Labeled with FITC.
Specificity：Polyvalent for Salmonella "O" & "H" antigens. Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae. Immunocaptures Salmonellae
Isotype：IgG
Calc Applications Abbrev：IF
Immunogen：Mixture of S. enteriditis, S. typhimurium & S. heidelburg
