Rotaviruses, members of the family Reoviridae, are a major cause of diarrhea in young mammals.
Human rotaviruses, the major etiological agents of severe infantile diarrhea worldwide, display very diverse and complex serotypic specificities.
Rotaviruses are 70nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2.
The immunity acquired from exposure to rotavirus appears to be type specific following initial infection.
Therefore, multiple serotypes of rotavirus mean multiple opportunities for infection.
The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunocytochemistry: 1:20-1:200Western Blot: 1:20-1:200ELISA: 1:200-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：goat
Grade：Highly Purified
Purity：Purified IgG fraction to a purity of > 95%.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.002% thimerosal. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes ICPs and late structural (virion) antigens. >90% with human rotaviruses (reported). Uninfected Cell Reactivity: Negative vs. HEp-2 cells and WI-38 cells by indirect immunofluorescence.
