Legionella pneumophila is the causative agent of the Legionnaires' disease, a potential fatal pneumonia.
It was isolated after an outbreak of pneumonia at an American legion convention in Philadelphia.
The strain Philadelphia 1 is derived from the original 1976 isolate.
Legionella pneumophila is found in biofilms and fresh and industrial water systems worldwide.
It can survive and replicate intracellularly in free-living protozoa, often in aquatic biofilms.
Applications:Suitable for use in Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: Acetone fixation of the antigen source is recommended prior to staining.
Optimal dilutions to be determined by the researcher.
Titer:>1:800 by Indirect IFA vs.
acetone-fixes organisms
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：rabbit
Grade：Highly Purified
Purity：Purified (>95%).
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes Legionella pneumophila serogroups 1-12. Recognizes all antigens of intact microorganism. Antiserum is not absorbed and may crossreact with related microorganisms.
Isotype：IgG
Calc Applications Abbrev：IF
Immunogen：A whole cell preparation of Legionella pneumophila; ATCC #33152.
