84-1064-72 Escherichia coli, O, K Serotypes (E. coli) (Biotin) 1ml E3500-06K

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  • Escherichia coli (E.
  • coli) is a Gram negative bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms).
  • Most E.
  • coli strains are harmless, but some, such as serotype O157:H7, can cause serious food poisoning in humans, and are occasionally responsible for costly product recalls.[1][2] The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2,[3] or by preventing the establishment of pathogenic bacteria within the intestine.[4][5] E.
  • coli are not always confined to the intestine, and their ability to survive for brief periods outside the body makes them an ideal indicator organism to test environmental samples for fecal contamination.[6][7] The bacteria can also be grown easily and its genetics are comparatively simple and easily-manipulated or duplicated through a process of metagenics, making it one of the best-studied prokaryotic model organisms, and an important species in biotechnology and microbiology.
  • E.
  • coli was discovered by German pediatrician and bacteriologist Theodor Escherich in 1885,[6] and is now classified as part of the Enterobacteriaceae family of gamma-proteobacteria.[8]
  • A strain of E.
  • coli is a sub-group within the species that has unique characteristics that distinguish it from other E.
  • coli strains.
  • These differences are often detectable only on the molecular level; however, they may result in changes to the physiology or lifecycle of the bacterium.
  • For example, a strain may gain pathogenic capacity, the ability to use a unique carbon source, the ability to inhabit a particular ecological niche or the ability to resist antimicrobial agents.
  • Different strains of E.
  • coli are often host-specific, making it possible to determine the source of fecal contamination in environmental samples.[6][7] For example, knowing which E.
  • coli strains are present in a water sample allows to make assumptions about whether the contamination originated from a human, another mammal or a bird.
  • Escherichia coli cells propel themselves with flagella (long, thin structures) arranged as bundles that rotate counter-clockwise, generating torque to rotate the bacterium clockwise.
  • E.
  • coli is Gram-negative, facultative anaerobic and non-sporulating.
  • Cells are typically rod-shaped and are about 2 micrometres (um) long and 0.5 um in diameter, with a cell volume of 0.6-0.7cm3.[9] It can live on a wide variety of substrates.
  • E.
  • coli uses mixed-acid fermentation in anaerobic conditions, producing lactate, succinate, ethanol, acetate and carbon dioxide.
  • Since many pathways in mixed-acid fermentation produce hydrogen gas, these pathways require the levels of hydrogen to be low, as is the case when E.
  • coli lives together with hydrogen-consuming organisms such as methanogens or sulfate-reducing bacteria.[10] Optimal growth of E.
  • coli occurs at 37°C but some laboratory strains can multiply at temperatures of up to 49°C.[11] Growth can be driven by aerobic or anaerobic respiration, using a large variety of redox pairs, including the oxidation of pyruvic acid, formic acid, hydrogen and amino acids, and the reduction of substrates such as oxygen, nitrate, dimethyl sulfoxide and trimethylamine N-oxide.[12] Strains that possess flagella can swim and are motile.
  • The flagella have a peritrichous arrangement.[13] E.
  • coli and related bacteria possess the ability to transfer DNA via bacterial conjugation, transduction or transformation, which allows genetic material to spread horizontally through an existing population.
  • This process led to the spread of the gene encoding shiga toxin from Shigella to E.
  • coli O157:H7, carried by a bacteriophage.[14]
  • Applications:Suitable for use in Indirect Immunofluorescence and Immunocytochemistry.
  • Other applications not tested.
  • Recommended Dilution:Optimal dilutions to be determined by the researcher.
  • Titer: > 1:1,600 by indirect IFA vs.
  • serotypes O157:H7, O20, O125, O55, O111 and K12
  • Storage and Stability:May be stored at 4°C for short-term only.
  • Aliquot to avoid repeated freezing and thawing.
  • Store at -20°C.
  • Aliquots are stable for 12 months after receipt.
  • For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.


  • Size:1ml
  • Host:goat
  • Grade:Highly Purified
  • Purity:Purified IgG fraction to a purity of >95%.
  • Form:Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins have been added. Labeled with Biotin.
  • Specificity:Recognizes many "O" and "K" antigenic serotypes of Escherichia coli. Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae.
  • Isotype:IgG
  • Calc Applications Abbrev:IC IF
  • Immunogen:Mixture of E. coli serotypes
  • Shelf Life:1year
  • EU Commodity Code:30021010
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アズワン品番 84-1064-72
型番 E3500-06K
入り数 1個
標準価格 122,000円(税抜)
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