Cholera toxin is comprised of two subunits, A and B.
Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration.
The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1.
Applications:Suitable for use in ELISA and Gel Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
End-Point Titer:>1:1000 by ELISA
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：rabbit
Grade：Highly Purified
Purity：Purified IgG fraction to a purity of ~95%.
Form：Supplied as a liquid in PBS, 10mg/ml BSA, 0.002% thimerosal. Labeled with HRP. Estimated molar HRP:IgG ratio: 2-3.
Specificity：Recognizes the beta subunit of Cholera toxin. Crossreacts with E. coli heat labile toxin. Immunoprecipitates toxin in gel techniques.
