84-1064-49　Cholera Toxin, beta (Cholera Enterotoxin beta, Choleragenoid, CTX B, CTXB, CTX-B, TOXB, VC1456) (FITC) 1ml　C4275-35P

特徴

• Cholera toxin is comprised of two subunits, A and B.
• Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration.
• The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1.
• Applications:Suitable for use in FLISA, Immunofluorescence, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:FLISA: >1:1,000Immunofluorescence: 1:10-1:50.
• Direct FA staining of target antigens in a permissive tissue culture system.
• Acetone fixation of the antigen source is recommended prior to staining.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.