The Beta subunit of cholera toxin binds to a GM1-ganglioside receptor which is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin Alpha subunit into cells.
The holotoxin consists of a pentameric ring of Beta subunits whose central pore is occupied by the Alpha subunit.
The Alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge.
The Alpha subunit (and Cholera toxin) activate the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.
Applications:Suitable for use in ELISA and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Titer: >1:1000 by ELISA
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：rabbit
Grade：Highly Purified
Purity：Purified IgG fraction to a purity of > 95%.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins have been added.
Specificity：Recognizes the Beta subunit of Cholera toxin. Cross reacts with E.coli heat liable toxin. Immunoprecipitates toxin in gel techniques.
