Chlamydia is caused by the bacterium Chlamydia trachomatis.
The intracytoplasmic inclusions caused by the bacterium are draped around the infected cell’s nucleus.
Chlamydia has a genome size of approximately 500-1000 kilobases and contains both RNA and DNA.
The organism is also extremely temperature sensitive and must be refrigerated at 4°C as soon as a sample is obtained.
Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia.
It persists at body sites that are inaccessible to phagocytes, T-cells and B-cells.
It also exists as 15 different serotypes.
These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K), and lymphogranuloma venereum (caused by serotypes L1, L2, and L3).
Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes.
Applications:Suitable for use in ELISA and Immunofluorescence.
It is also suitable for Conjugation purposes.
Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Titer:>1:1,000 by indirect Immunofluorescence vs.
all serovars (A-K, L1-L3)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:1ml
Host:goat
Grade:Highly Purified
Purity:Purified IgG fraction to a purity of > 95%.
Form:Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins have been added.
Specificity:Recognizes Major Outer Membrane Protein (MOMP). Recognizes the following serovars: A, B, Ba, C, D, E, F, G, H, I, J, K, L1, L2, L3. Does not react with C. psittacii or C. pneumoniae in MIF. Uninfected Cell Reactivity: Negative vs. HEp-2 cells and egg yolk sac.
Isotype:IgG
Calc Applications Abbrev:E IF
Immunogen:Chlamydia trachomatis strain L2, purified Major Outer Membrane Protein (MOMP)