84-1064-39　Chlamydia trachomatis L2 (FITC) 1ml　C4250-52C

特徴

• Chlamydia is caused by the bacterium Chlamydia trachomatis.
• The intracytoplasmic inclusions caused by the bacterium are draped around the infected cell’s nucleus.
• Chlamydia has a genome size of approximately 500-1000 kilobases and contains both RNA and DNA.
• The organism is also extremely temperature sensitive and must be refrigerated at 4°C as soon as a sample is obtained.
• Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia.
• It persists at body sites that are inaccessible to phagocytes, T-cells and B-cells.
• It also exists as 15 different serotypes.
• These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K), and lymphogranuloma venereum (caused by serotypes L1, L2, and L3).
• Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes.
• The cell wall is proposed to be gram-negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall.
• This lack of peptidoglycan is shown by the inability to detect muramic acid and antibodies directed against it.
• It may, however, contain a carboxylated sugar other than muramic acid.
• The proposed structure consists of a major outer membrane protein cross-linked with disulfide bonds.
• It also contains cysteine-rich proteins (CRP) that may be the functional equivalent to peptidoglycan.
• This unique structure allows for intracellular division and extracellular survival (Hatch 1996)
• -Kenneth Todar, University of Wisconsin-Madison
• Applications:Suitable for use in Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:10-1:50.
• Direct FA staining of target antigens in a permissive tissue culture system.
• Acetone fixation of the antigen source is recommended prior to staining.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.