Borrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals.
B.
burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response.
B.
burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen.
B.
burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.
Applications:Suitable for use in ELISA, Immunohistochemistry, Fluorescence Microscopy, Western Blot and Immunocytochemistry.
It is also suitable for Conjugation purposes.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Formalin-fixed paraffin-embedded sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:1ml
Host:rabbit
Grade:Highly Purified
Purity:Purified IgG fraction to a purity of ~95%.
Form:Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins have been added.
Specificity:Recognizes Borrelia burgdorferi at 83kD, 41kD, 34kD and 31kD, and additional low MW bands. Crossreactivity: Treponema pallidum, B. hermsii & B. parkerii.
Isotype:IgG
Calc Applications Abbrev:E IC IF IHC WB
Immunogen:Whole cell preparation from B. burgdorferi