TLR4 (Toll-like receptor 4) is a mammalian homologue of the Drosophila Toll protein.
The human TLR4 is a 799-residue type-1 transmembrane protein.
The extracellular domain has leucine-rich repeats and the intracellular domain is similar to that of the IL-1 receptor.
TLR4 is a receptor for lipopolysaccharide (LPS).
In mice that are genetically tolerant to endotoxin, mutations have been identified in the intracellular domain of TLR4 that abolish LPS responsiveness.
TLR4 uses a mechanism similar to that of the IL-1 receptor for signal transduction that leads to activation of NF-kB.
Applications:Suitable for use in Neutralizing, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.09% sodium azide.
Specificity：Reacts with the TLR4 protein.
Isotype：IgG
Calc Applications Abbrev：FC IHC IP N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa6-169 in human TLR4 expressed in E. coli.
