84-1064-16　RGS3 (Regulator of G protein signaling 3) 200ug　R1995-15A

特徴

• RGS3 (regulator of G protein signaling 3) is a 519-residue protein that is widely expressed.
• The protein contains an RGS domain near the carboxy terminus that serves as a GTPase-activating protein (GAP).
• RGS3 negatively regulates the activation of Gi and Gq.
• A truncated RGS3 (RGS3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC.
• RGS3T may be localized in the nucleus and RGS3 is found in cytoplasm.
• Translocation to membrane is important for the RGS proteins to interact with heterotrimeric G proteins.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
• Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.