NIK (NF-kB-inducing kinase) is a member of the MAP kinase family that binds TRAF2 and stimulates NF-kB activity.
NIK, initially isolated from a human B cell cDNA library, contains 795 amino acids with an apparent molecular weight of slightly more than 97kD on SDS gel.
NIK is a serine/threonine kinase and its kinase activity contributes to IkB phosphorylation.
The carboxyl terminal segment of NIK binds TRAF2.
A mutant NIK with intact carboxyl terminus but without the two lysine residues at its catalytic domain serves as a dominant-negative inhibitor for NF-kB activation.
NIK also interacts with TRAF6 and mediates IL-1-induced NF-kB activation.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000Immunoprecipitation: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 40% glycerol.
Specificity：Reacts with human NIK.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：E. coli-expressed human NIK fragment (aa795-953)
