Rat MCP-1/JE (monocyte chemoattracctant protein-1) is an 148-amino acid CC chemokine with a NH2-terminal sequence of 29 residues as a signal sequence.
It was originally cloned from Con A-stimulated rat spleen cDNA library.
This rat MCP-1/JE is 49-amino acid longer than human MCP-1 at 3'-end.
This 3'-end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and which explains for the higher molecular weight (25 kD).
In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophils.
MCP-1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：250ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.09% sodium azide, before the addition of glycerol to 40%.
Specificity：Recognizes both mouse JE and rat MCP-1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo Rt
Immunogen：E. coli-expressed N-terminal amino acid 1-73 of rat MCP-1
