Fractalkine, also known as neurotactin, is a membrane-bound CX3C chemokine.
The mature protein is part of a 397-amino acid precursor and consists of a chemokine domain of 76 amino acids, a mucin stalk of 241 amino acids, a putative transmembrane domain of 18 amino acids, and an intracellular tail of 37 amino acids.
Within the chemokine domain the first two cysteine residues are separated by 3 amino acids (CX3C).
Fractalkine message is found in high abundance in the brain, kidney, lung and heart tissues.
Fractalkine is chemotactic for monocytes and other leukocytes including NK cells, lymphocytes, and may play a role in brain inflammation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder in 0.09% sodium azide.
Specificity：Reacts with recombinant and natural human fractalkine.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：E. coli-expressed intracellular domain (aa 379-397) of human fractalkine
