Fractalkine, also termed a 397-amino acid precursor consisting of a chemokine domain (76 amino acids), a mucin stalk of 241 residues, a putative transmembrane domain (18 amino acids), and an intracellular tail of 37 amino acids.
Within the chemokine domain the first two cysteine residues are separated by 3 amino acids.
Fractalkine message is found at high concentrations in the brain, and also in kidney, lung and heart.
Fractalkine is chemotactic for monocytes and may play a role in brain inflammation.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:2000Immunoprecipitation: 1:300-1:800Immunohistochemistry: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder in 0.09% sodium azide.
Specificity：Reacts with recombinant and natural mouse fractalkine. Species Crossreactivity: <30% cross-reactivity to human and rat Fractalkine.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Mo
Immunogen：E. coli expressed chemotactic domain of mouse fractalkine
