DAP12 (KARAP) is a natural killer (NK) cell-expressed cell surface receptor and belongs to the immunoglobulin and C-type lectin superfamily.
Unlike other members of this family which possess immunoreceptor tyrosine-based inhibitory motifs (ITIM), DAP12 contains an immunoreceptor tyrosinebased activation motif (ITAM) in its cytoplasmic domain and is a disulfide-bonded homodimer.
Crosslinking of DAP12-KIR complexes (membrane glycoproteins of the killer-cell inhibitory receptor family without an ITIM in their cytoplasmic domain) results in cellular activation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a lyophilized powder in 0.09% sodium azide.
Specificity：Reacts with both recombinant and natural rat DAP12.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rt
Immunogen：E. coli-expressed rat DAP12 peptide (amino acid 28-40)
