Fractalkine, also termed neurotactin, is a membrane-bound CX3C chemokine1.
human CX3CR1, also named V28, was first cloned from human genomic DNA libraries.
A G-protein-coupled seven-transmembrane domain receptor, was recently identified to serve as fractalkine receptor.
Human CX3CR1 has been shown to mediate both the adhesive and migratory functions of fractalkine.
Fractalkine and CX3CR1 represent new types of leukocyte trafficking regulators.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:2000Immunoprecipitation: 1:300-1:800Immunohistochemistry: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS with 0.09% sodium azide.
Specificity：Reacts with recombinant and natural human and rat CX3CR1.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Rt
Immunogen：E. coli-expressed human CX3CR1 amino terminal domain
