The vesicular monoamine transporter is responsible for the vesicular uptake of monoamines, like dopamine, norepinephrine, epinephrine, serotonin and histamine.
Applications:Suitable for use in Immunohistochemistry, Immunofluorescence Microscopy and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Frozen): 1:2000-1:3000 using FITC secondary antibody; Incubation overnight at 4°C.
10-100ug immunogen per ml diluted antiserum abolishes staining
Immunofluorescence Microscopy: 1:2000-1:3000Western Blot: 1:500; Using a crude synaptic vesicle fraction of rat brain, a 70kD band is recognized
Optimal dilutions to be determined by the researcher.
Recommended Positive Control:IHC-T5595-7163: Rat small intestine
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：guinea pig
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O. Dilute further with PBS, 1% BSA and 0.09% sodium azide.
Specificity：Recognizes rat monoaminergic neurons of the CNS, the ECL-cells of the stomach, as well as enteric nerve fibers. Species Crossreactivity: Human mouse and porcine.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Po Rt
Immunogen：Synthetic peptide (CQSYPIGDDEESESD-OH) corresponding to the C-terminus of rat VMAT2 (BSA).
