The antiserum against the vesicular acetylcholine transporter is a unique immunohistochemical marker for cholinergic nerves, more specific than the commonly used acetylcholinesterase (AchE), since it does not react with postsynaptic neurons, and is more sensitive than choline acetyltransferase (ChAT).
Applications:Suitable for use in Immunohistochemistry (frozen sections).
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:800-1:1200 using FITC conjugates as secondary antibodies; Incubation Time Overnight at 2-8°C
Optimal dilution determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder.
Specificity：Recognizes human VAChT both in the CNS and PNS. Absorption with 10-100ug immunogen per ml diluted antiserum abolishes staining. Recommended for Frozen sections of human small intestine.
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide from the C-terminus of human VAChT conjugated to BSA.
