The antiserum against the vesicular acetylcholine transporter is a unique immunohistochemical marker for cholinergic nerves, more specific than the commonly used acetylchiolinesterase (AchE), since it does not react with postsynaptic neurons, and is more sensitive than choline acetyltransferase (ChAT).
Applications:Suitable for use in Immunohistochemistry (frozen sections).
Recommended Dilution:Immunohistochemistry: 1:5000-1:10,000 using FITC; Incubation Time Overnight at 4°C.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：guinea pig
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder.
Specificity：Recognizes VAChT both in the CNS and PNS of rat and mouse. Absorption with 10-100ug immunogen per ml diluted antiserum abolishes staining. Recommended for Frozen sections of rat small intestine. Species Crossreactivity: Rat, mouse.
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide from the C-terminus of rat VAChT conjugated to BSA.
