84-1063-38　Plectin 100ul　P4214-31

特徴

• Plectin, a 500kD intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking (1).
• Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes (2,3).
• Plectin binds many proteins, including actin, vimentin, and Lamin B.
• Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions (4).
• Plasma membrane attachment sites of intermediate filaments and microfilaments (actin) such as desmosomes and hemidesmosomes, Z-line structures and dense plaques of striated and smooth muscle.
• Strong positive reaction is also found with endothelia, fibroblasts and infiltrating cells (e.g.
• lymphocytes).
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:16,000 (ECL)Immunohistochemistry (frozen and paraffin sections, enhanced after microwave treatment): 1:500: Incubate for 1 hr at RT; extended with paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.