The predominant forms in adipocytes, perilipin A and B arise by alternative RNA splicing from a single gene, generating polypepides of 57 and 46kD, respectively.
The N-terminus, however, remains unchanged.
The antiserum recognizes perilipins (A and B) located at the surface of intracellular storage lipid droplets present, e.g.
in the adrenal gland, adipocytes of white and brown adipose tissue, and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells.
Applications:Suitable for use in Western Blot and Immunohistochemistry Other applications have not been tested.
Microwave treatment recommendedWestern Blot: 1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：guinea pig
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.09% sodium azide.
Specificity：Recognizes perilipins (A and B) at ~57 and ~46kD, respectively. Does not cross-react with ADRP (adipophilin) or TIP47 proteins. Species Crossreactivity: human, rat and mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Duplicated N-terminus corresponding to aa1-20 MSMNKGPTLLDGDLPEQENV-C of PL1N1.
